What We Do
We study the development and function of the mammalian immune system at a single-cell level. Our goal is to understand the differences between the immune cells that develop preferentially in infants versus adults, and determine the functional impact of these differences in promoting host immunity or immunopathology. Our studies employ and drive the frontiers of high-dimensional (18+ parameter) flow cytometry technologies to isolate, characterize, and transplant single immune and stem cells from both fetal, neonatal, and adult tissues.
Eliver Ghosn
Assistant Professor of Medicine
Lowance Center for Human Immunology
Health Sciences Research Building
1760 Haygood Dr., Office E240
Phone: (404) 712-3211
Lab Goals
Our studies, in both mice and humans, will provide new insights into the development and function of the human immune system in infants and adults and inform the development of new vaccines that are targeted to either children or adults. In addition, the results from our studies will shed light on the mechanisms that lead to autoimmunity and other hematological disorders (leukemia/lymphoma, immunodeficiency, etc.) that are predominant at different developmental ages.
News
Publications
- Fetal Hematopoietic Stem Cell Transplantation Fails to Fully Regenerate the B-Lymphocyte Compartment (Published in Stem Cell Reports)
- Fetal Hematopoietic Stem Cell Transplantation Fails to Fully Regenerate the B-Lymphocyte Compartment (Published in Stem Cell Reports)
- Hematopoietic stem cell–independent B-1a lineage (Published in the Annals of NY Academy of Sciences)
- Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells (Published in PNAS)
Merinoff World Congress 2014: B-1 Cell Development and Function
After a long hiatus of 23 years since the last B-1 Congress, Dr. Ghosn, together with Drs. Rothstein and Holodick (Western Michigan), co-organized the first international B-1 Congress that brought together more than 150 participants in New York to discuss new discoveries in B-1 cell biology. The congress featured eminent immunologists in the field of B cells and included a keynote address, the Herzenberg Lecture, by Dr. Klaus Rajewsky.
Publications
Under Review
1. Ghosn EE, Kosuge H, Larson T, Herzenberg LA, Gambhir SS, McConnell MV, Smith BR. Carbon nanotubes for selective photoacoustic imaging of atherosclerotic macrophages.
2. Orlova D, Meehan S, Moore W, Parks D, Meehan C, Dill D, Zimmerman N, Ghosn EE, Herzenberg LA, Walther G. Science not art: statistically sound methods for defining subsets in large multi-dimensional datasets.
3. Orlova D, Meehan S, Parks D, Moore W, Meehan C, Ghosn EE, Herzenberg LA, Walther G. QFMatch: multidimensional flow and mass cytometry samples alignment.
Published
1. Min J, Raju P, Hulse K, Stevens W, Huang J, Van Roey G, Norton J, Suh L, Carter R, Price C, Weibman A, Rashan A, Ghosn EE, Homma T, Conley D, Welch K, Shintani-Smith S, Peters A, Grammer III L, Harris K, Kato A, Hwang P, Kern R, Herzenberg L, Schleimer R, Nayak J, Tan B. Evidence for altered levels of IgD in the nasal airway mucosa of patients with chronic rhinosinusitis. JACI in press 2017
2. Ghosn EE*, Waters, J, Phillips M, Yamamoto R, Long B, Yang Y, Gerstein R, Stoddart C, Nakauchi H, Herzenberg, LA. Fetal hematopoietic stem cell transplantation fails to fully regenerate the B-lymphocyte compartment. Stem Cell Reports 2016 *Corresponding Author | Recommended in F1000 prime
3. Orlova D, Zimmerman N, Meehan S, Meehan C, Waters J, Ghosn EE, Filatenkov A, Kolyagin G, Gernez Y, Moss R, Herzenberg LA, Walther G. Earth Mover's Distance (EMD): a true metric for comparing biomarker expression levels in cell populations. PLoS One 2016
4. Rothstein TL, Herzenberg LA, Holodick NE, Ghosn E. B-1 Cell Development and Function. Ann NY Acad Sci. 2015
5. Ghosn EE* & Yang Yang. Hematopoietic stem cell-independent B-1a lineage.Ann NY Acad Sci. 2015Corresponding Author
*Corresponding Author
6. Rashan A, Ghosn EE, Peterson A, Yang Y, Phillips M, Sahaf B, Herzenberg LA, Nayak JV. Characterization of immunoglobulin E plasma cells that are elevated in the upper airway mucosa of nonatopic patients with chronic rhinosinusitis without nasal polyps. Int Forum Allergy Rhinol. 2016
7. Kurnellas MP, Ghosn EE, Schartner JM, Baker J, Rothbard J, Negrin R, Herzenberg LA, Fathman CG, Steinman L, Rothbard JB. Amyloid Fibrils Activate B-1a Lymphocytes to Ameliorate Inflammatory Brain Disease. PNAS. 2015
8. Yang Y, Wang C, Yang Q, Kantor AB, Chu H, Ghosn EE, Qin G, Mazmanian SK, Han J, Herzenberg LA. Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires. eLife 2015
9. Smith B, Ghosn EE, Rallapalli H, Prescher J, Herzenberg LA, Gambhir SS. Selective uptake of single-walled carbon nanotubes by circulating monocytes for enhanced tumour delivery. Nature Nanotech. 2014
10. Meehan S, Walther G, Moore W, Orlova D, Meehan C, Parks D, Ghosn EE, Philips M, Mitsunaga E, Waters J, Kantor A, Okamura R, Owumi S, Yang Y, Herzenberg LA and Herzenberg LA. AutoGate: automating analysis of flow cytometry data. Immunol Res. 2014
11. Araujo T, Okada S, Ghosn EE, Taniwaki NN, Rodrigues MR, Almeida SR, Mortara RA, Russo M, Campa A, Albuquerque RC. Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages. Cell Immunol. 2013
12. Grant J, Ghosn EE, Axtell RC, Herges K, Kuipers HF, Woodling NS, Andreasson K, Herzenberg LA, Herzenberg LA, Steinman L. Reversal of Paralysis and Reduced Inflammation from Peripheral Administration of β-Amyloid in TH1 and TH17 Versions of Experimental Autoimmune Encephalomyelitis. Sci Transl Med. 2012
*Selected for the magazine cover
13. Ghosn EE*, Yamamoto R, Hamanaka S, Yang Y, Herzenberg LA, Nakauchi H, Herzenberg LA. Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells. PNAS. 2012
*Corresponding Author | Highlighted in In This Issue of PNAS
14. Kusy S, Ghosn EE, Herzenberg LA, Contag CH. Development of B cells and erythrocytes is specifically impaired by the drug celastrol in mice. PLoS One 2012
15. Yang Y, Ghosn EE, Cole LE, Obukhanych TV, Sadate-Ngatchou P, Vogel SN, Herzenberg LA, Herzenberg LA. Antigen-specific memory in B-1a and its relationship to natural immunity. PNAS. 2012
16. Yang Y, Ghosn EE, Cole LE, Obukhanych TV, Sadate-Ngatchou P, Vogel SN, Herzenberg LA, Herzenberg LA. Antigen-specific antibody responses in B-1 and their relationship to natural immunity. PNAS. 2012
17. Cassado A, de Albuquerque J, Sardinha L, Buzzo CL, Faustino L, Nascimento R, Ghosn EE, Lima MR, Alvarez JM, Bortoluci KR. Cellular renewal and improvement of local cell effector activity in peritoneal cavity in response to infectious stimuli. PLoS One 2011
18. Ghosn EE*, Sadate-Ngatchou P, Yang Y, Herzenberg LA, Herzenberg LA. Distinct progenitors for B-1 and B-2 cells are present in adult mouse spleen. PNAS. 2011
*Corresponding Author
19. Gernez Y, Tirouvanziam R, Yu G, Ghosn EE, Reshawmala N, Nguyen T, Tsai M, Galli SJ, Herzenberg LA, Nadeau KC. Basophil CD203c levels are increased at baseline and can be used to monitor omalizumab treatment in subjects with nut allergy. Int Arch Allergy Immunol. 2011
20. Ghosn EE*, Cassado A, Govoni G, Fukuhara T, Yang Y, Monack D, Bortoluci K, Almeida SR, Herzenberg LA. Two physically, functionally and developmentally distinct peritoneal macrophage subsets. PNAS. 2010
*Corresponding Author
21. Sousa MG, Ghosn EE, Nascimento RC, Bomfim GF, Noal V, Santiago K, Silva Azevedo CM, Marques SG, Gonçalves AG, Lima Santos DW, Criado PR, Costa Martins JE, Almeida SR. Monocyte-derived dendritic cells from patients with severe forms of chromoblastomycosis induce CD4+ T cell activation in vitro. Clin Exp Immunol. 2009\
22. Ghosn EE, Yang Y, Tung J, Herzenberg LA. CD11b expression distinguishes sequential stages of peritoneal B-1 development. PNAS. 2008
23. Sousa MG, Azevedo C, Nascimento R, Ghosn EE, Santiago KL, Noal V, Bomfim GF, Marques SG, Gonçalves AG, Wagner de Castro D, Almeida SR. F. pedrosoi infection induces differential modulation of co-stimulatory molecules and cytokines in monocytes from patients with severe and mild forms of chromoblastomycosis. J Leukoc Biol. 2008
24. Yang Y, Tung J, Ghosn EE, Herzenberg LA. Division and differentiation of natural antibody-producing cells in mouse spleen. PNAS. 2007
25. Sousa MG, Ghosn EE, Almeida SR. Absence of CD4+ T cells impairs host defense of mice infected with Fonsecaea pedrosoi. Scand J Immunol. 2006
26. Hayakawa M, Ghosn EE, Sousa MG, Ferreira KS, Almeida SR. Phagocytosis, production of nitric oxide and pro-inflammatory cytokines by macrophages in the presence of dematiaceous fungi that cause chromoblastomycosis. Scand J Immunol. 2006
27. Ghosn EE, Russo M, Almeida SR. Nitric oxide-dependent killing of Cryptococcus neoformans by B-1 derived mononuclear phagocyte. J Leukoc Biol. 2006
Collaborations
In addition to our main research projects, we have ongoing collaborations with laboratories in the US and abroad:
- Nakauchi Lab at University of Tokyo and Stanford University
- Roan Lab at University of California - San Francisco
- Mellins Lab at Stanford University
- Smith Lab at Stanford University
Join Our Team
We are currently recruiting postdocs and research specialists. To apply, send your CV, a list of three references, a letter describing your previous experience, and a brief statement on why you are interested in our work to Eliver Ghosn. We look forward to hearing from you!
Available Positions
The Ghosn lab is looking to recruit highly enthusiastic researchers interested in examining the development and function of the mammalian immune system at a single-cell level. The new team member will receive advanced training in high-dimensional (18+ parameter) flow cytometry techniques and participate in research at the frontier of this and related technologies, including high-throughput single cell-based gene expression assays (HT-qPCR and RNA/BCR sequencing).